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Intestinal Parasites: Flat worms, Round worms
Flat Worms
Introduction

Tape worms
Is a type of flat worm that belongs to cestoda class (cestodes). They consist of a number of sections occurring one after the other. Each section contains separate reproductive organs but the nervous and excretory systems are continous through the whole worm. The head part is called the scorlex and consists of the hooks, or suckers. The separate sections also called proglotides bud off from the scorlex one by one to make a chain called strobila. The sections furthest from the head are the oldest. The infection of the tape worm (taeniosis) affects almost all domestic and wild animals. Birds, fishes, amphibian, reptiles and certain worms are also affected. In livestock, the cestode/tape worm parasitic occurs in either adult or larval stages


Life cycle of round tape worms
The general life cycle of tape worms occurs in two hosts, the final and the intermediate hosts. The eggs of adult worm are fertilized and develop into embryos in the uterus within a segment (proglottis). The segment will break off from the rest of the chain and are passed out of the body of the host in feces after degeneration of proglottis to form a sac of embyonated eggs. Within the egg of all tape worms, an embryo develops which is eaten by the intermediate host where it transforms into a bladder like structure. The embryo may then transform into one big bladder like structure with numerous invaginated walls called coenurus. The embryo may also transform into one bladder like structure with one invagination in the wall called cystercus. In Echnicoccus granulosis species of tape worm, the bladder like structure may bud off to form daughters and grand daughters of called hydatid cyst.
The coenurus, cystercus and hydatid cysts will each produce at the base the scorlex including the hooks which will become the scorlex of the future worm in the final host. It is the larval stage which enters the intermediate host and the invaginated head remains until when the intermediate host is taken up into the body of the final host. The invaginated head fixes itself into the tissues of the final host to begin a new adult phase.
In their life cycle, tape worms are able to develop in a variety of intermediate hosts and the intermediate hosts are likely to beaten by the final host. The adult stage of the tape worm is often host specific. The effects of tape worms are wide spread in almost all tropical countries. The animals may carry a moderate or heavy infestation without showing ill health. However, infected calves will have stunted growth and may die.



Some important adult tape worms of cattle

1. Monieza benedeni
It affects ruminants, and mainly cattle. It is present in most parts of the world. The life cycle is through oribatid (beetle) mites. The eggs or shed proglottides containing eggs are eaten by the final host in 2 - 6 months. Mites are present in grazing pastures in large numbers. The mites are ingested by grazing animals and larvae develop in the intestine into adult tape worms in about 6 weeks. The adult tape worm lives for about 3 months in the final host.

2. Avittelina species
They occur in the small intestines of cattle and other ruminants. They are present mainly in Africa and India. The adult tape worm measures about 3 meters long and 3mm wide.

3. Thysanieza giardi
Occurs in the small intestine of cattle, sheep and goats and are mainly present in Africa. The life cycle is through the oribatid beetle.



Clinical signs and diagnosis
The effect of adult tape worms in cattle may not be great. However, heavy infestation may cause disease especially in the presence of other stress factors like nutritional deficiencies or bacterial infections.
The clinical effects include stairing coat, digestive disturbance, pot belly, anemia and oedema. When present in large numbers, tape worms can cause blockage in the intestines. Diarrhea and constipation may sometimes be observed.


Diagnosis
Is made from the presence of the tape worm segments found in the feaces or intestines or from the presence of the tape worm eggs in the feaces. The presence of the eggs in the feaces would be demonstrated by direct smear or by the floatation technique.


Post mortem examination
  • Tape worms would be present in the intestines or in the bile duct
  • The carcass would be pale and emaciated
  • The walls of the intestines would show ulcerations with inflammation on the wall where the hooks of tape worms has caused a reaction
  • Presence of segments in feaces



Prevention - Control - Treatment

Control /preventive measures
  • Pasture containing mites should be ploughed or cultivated to reduce the number of mites. This is only a temporary control measure.
  • Use of taenicide drugs (see treatment drugs above) is the most important effective control method.


Treatment The following are some of the available effective drugs that can be used for treatment of tape worms:
  • Copper II sulphate mixed with nicotine sulphate used at a dose arte of 150ml - 280 mls
  • Niclosanmide: other trade names Mansonil, Yonesan, Lintex can be used at a dose rate of 100mg/kg. This drug is not recommended for lactating cows.


Larval tape worm infection in cattle
Most of the tape worms causing larval infection to livestock belong to the family Taeniidae of the order cyclophyllidae. The definitive hosts are mainly canine and humans who are also affected by adult parasites. The taeniidae also affect various domestic animals. Some tape worms that affect cattle and humans include the following: taenia siginata, taenia hydatigena, Taenia multiceps and Echinococcus granulosus.


1. Taenia siginata
It is foud in the intestines of humans and measures about 4 - 15 m long. The gravid segments are 15 - 20 mm long and 4 - 7 mm wide. Each segement contains about 100,000 eggs. The strobila may have about 2000 proglottides. The active proglottides are shed and can move some distance away from the human host. The proglottide produces milky fluid containing tiny eggs. The eggs are ingested by cattle (primary host) where the embryos enter the blood stream and settle in the muscle tissues of the host thus causing a condition known as beef measles. The cysticercus bovis appears at meat inspection or post mortem as peaqrly white oval nodules observable by naked eyes. The cysticercus bovis has a life span of 2 - 3 years in human (secondary host). The humans are infected by eating raw or partially cooked beef.


2. Taenia hydatigena
The larval stage is found in the peritoneal cavity of most domestic animals including cattle. Embryos hatch in the intestines of the intermediate host and reach the liver via the blood stream. In the liver, they cause extensive damage. They then break out of the liver into the peritoneal cavity. The final host (dog) is affected by ingestion of the cysticercus.


3. Taenia multiceps
This is found in dogs as the definitive host. The larval stage is coenurus cerebralis and occurs in the brain and spinal cord of cattle and other intermediate hosts. Eggs are ingested by the intermediate host and embryos hatch in the intestines. They pass into the blood stream and localize in various parts of the body including the nervous system where they settle and develop to maturity. The final hosts become affected by eating coenurus in the brain or spinal cord of the infected intermediate host.


4. Echinococcus granulosus
This is a very small tape worm which has only 4 proglottides. It is found in dogs as the primary host. The larval stage (hydatid cyst) is found in numerous intermediate hosts including human, cattle and other domestic animals. Eggs are taken up by ingestion and embryos enter the blood stream and are carried to all parts of the body. The most commonly affected organs are the liver and the lungs. Six months after original infection, the inner lining of the hydatid buds off small vesicles called brood capsules which are similar with the hydatid. The brood capsules later develop into adult tape worm. The development of further cysts from the hydatid or the brood capsule may be accelerated by the rapture of the hydatid cyst or trauma. The final host is infected by ingestion of the fertile cysts and the worm arising from the scolex matures in 6 - 7 months.


Symptoms or clinical signs
Symptoms observed in the intermediate host vary depending on the organ of the body that is affected.
Cysticercus bovis has no apparent effect in the bovine host. However, Coenurus celebralis infection in ruminants is characterized by brain symptoms which depend on the position of the parasite in the brain or the spinal cord. The first symptoms after the first few weeks may include: High fever, inflammation of the brain and meningitis. The pressure exerted by the hydatid cyst may cause blindness, cyclic movement, jerky staggering gait, paralysis and prostration. Other general signs may include: grinding of teeth, salivation, lack of balance and convulsion. Pressure on the lumbar region of the spine may show signs of paralysis of the hind legs. The animal develops lack of appetite and become emaciated and dies from prostration. The effect of the hydatid cyst on the intermediate host depends also on the organ in which the cyst is situated. Some effects may include: Dysponea especially when the cyst is located in the lung, digestive upsets and ascites arising from liver infection. The main significance of the hydatid infection is that the animals act as reservoirs for human infection.


Postmortem/Necropsy
The lesions are more likely to be found as follows during meat inspection or at postmortem: Cysticercus bovis will be found embedded in the following parts: heart, tongue, diaphragm and masseter muscles.
Cysticercus celebralis: the animals are normally emaciated and anemic during meat inspection. Coenurus may also be found in the brain or spinal cord. The hydatid infection will reveal typical cysts in the lungs and liver.


Diagnosis
Accurate diagnosis can be done from samples collected by qualified personnel and analyzed at the laboratory.


Treatment
Treatment for larval stage of taeniids is not feasible because there are no suitable drugs or chemical compounds for their treatment.


Prevention and control
The principle of a proper control program aims at the following:
  • Breaking of the life cycle by treatment of the definitive host (human or any other host)
  • Destruction of tissues of the intermediate host containing cysts
  • Destruction of eggs during the passage from definitive host to intermediate host

The most effective control method should ensure the following:
  • Treatment of humans and dogs with taenicdes
  • Good meat inspection procedures by qualified meat inspectors and destruction of infected tissues or meat
  • Educating the public about the dangers of eating raw or partially cooked meat. The meat to be eaten by humans should be cooked at temperatures above 60 celsius degree or be frozen below 10 celsius degree for 10 days. These treatment methods are enough to destroy the cysts.
  • Use of pit latrines to keep the human waste away from the other animals. The public should be enlightened about the dangers of using human waste as fertilizer.
  • Fencing of slaughter houses with dog proof fences will keep stray dogs away and prevent them from eating condemned meat that may be infected with cysts.
Round Worms
Introduction

They cause round worm infection, or nematodiasis or parasitic gastro-enteritis The round worms contribute major economic loss in livestock production in the world. They are generally round in cross section, cylindrical, pointed at either ends and have smooth white cuticle. Each individual worm is male or female. They vary in length from 1 mm to 0.5 m. Their body structure comprise: The alimentary canal which starts with the mouth and is followed by the intestines and ends in the rectal opening near the tail. The worm feed through the mouth and pass the feed to the intestines where nutrients are absorbed through the intestinal wall. The reproductive organ of the male worm consists of along tube through which the spermatozoa are pumped. The female worm has the ovary, uterus and others at the same position as that of male. Male worms are assisted in copulation by the spicules which they use for attachment to the female. The spicules also direct the flow of sperms. In all cases, the male worm is smaller than the female.


Life cycle of round worms The round worms have two types of life cycle:
direct and indirect life cycles
In the direct life cycle, eggs hatch on the pastures into free living larvae which enter the host via the mouth or through the skin. In some cases, the eggs are picked by the host. In the in-direct life cycle, the eggs hatch into larvae then the larvae enters the intermediate host. Then the intermediate host is eaten by the final host. Sometimes, the eggs may be ingested by the intermediate host which is later taken by the final host. The larvae may as well penetrate through the skin of the host and later sucked by blood sucking intermediate host inside which they develop. The final host will then become infected by the bite of the intermediate host. In the life cycle of round worms, the intermediate host is always an invertebrate.



Nematode parasites of cattle

1. Neoascaris vitulorum
Is a type of round worm which occurs in the intestines of cattle and is present in most tropical countries. The worm has a thin translucent cuticle. Infection is more serious in calves than in mature cattle. Prenatal infection is very common. The worm has a direct life cycle whereby the eggs are swallowed and hatch inside the host. The larvae penetrates the intestinal wall and migrates to other tissues of the host especially the lung via the blood stream. The larvae would be coughed and then is reswallowed and then reenters then intestines. The larvae moult and become adult worms in the intestines where they produced eggs.


2. Haemonichus contortus
This is also called the large stomach worm. It is a common stomach worm of cattle and other ruminants. It is mainly found in the abomasums. The worm is distributed all over the world. It has a direct life cycle whereby the eggs are passed each day by worm and cab be observed in feaces. The larvae are ingested by the host and migrate in blades of grass when the grass is wet. Infective larvae can survive for several weeks in pasture under suitable climatic conditions. After the larvae are ingested by the host, they settle in the mucosa of the abomasums and pass through the fourth larval stage and become adult. The adult worms mate and the female worm pass eggs in the feaces of the final host.


3. Trichostrongylus species
There are several species of this worm widely spread in the tropics. The worm is brownish, pinkish in colour and is found in the abomasums and small intestines of cattle and other species of domestic animals including human. They have a direct life cycle similar with Haemonicus contortus. Infective larvae are ingested and settle in the alimentary canal of the host.


4. Cooperia species
These are small and red colored worms found in the small intestines of most ruminant animals. They have a direct life cycle similar with Trichostrongilus.


5. Ostertagia species
They are mainly found in the abomasums and occasionally in the small intestines of ruminants. They have a dire ct life cycle and are able to survive longer outside the host even under harsh conditions.They normally infect pasture during the onset of the long rains.


6. Nematodirus species
They are found in the small intestines of ruminant animals. The eggs develop outside the host and the larvae hatch at the infective stage. Te larvae develop to maturity inside the host.


7. Chabertia species
They are also known as the large mouthed bowel worms. They are found in the colon of most ruminants. They have a direct life cycle and infection of the host is by ingestion of the infective larvae. They larvae penetrate and embed in the wall of the colon. The adult worm passes eggs in feaces of the host.



Signs of Round worms

General clinical signs of round worms infection
However little the amount of worms may be, the host animal will appear stunted and emaciated due to competition on nutrients by the parasites.
The most common likely signs of an animals suffering from round worm burden are:
  • Lack of appetite, unthriftness, diarrhea, stairing coat, dehydration, pale mucus and anemia.
Gastro enteritis sings are common in the species of worms which affect the intestines and bronchitic signs would appear in the worms which affect the lungs. The bronchitic symptoms would be accompanied by coughing. In almost all cases of round worm infestation, the symptoms would be more pronounced in calves than older animals. Death would occur as a general outcome depending on the worm load and the type of treatment.


Necropsy/postmortem
  • The mucus membranes are often pale and the carcass is always emaciated.
  • The liver is pale and fragile
  • The alimentary tract will reveal worms when opened
  • The intestinal contents are usually reddish brownish in color.


Diagnosis
Detailed diagnosis should be done by a qualified personnel to identify the type of worm that is in occurrence and should follow the normal diagnostic procedures.

Prevention - Control - Treatment

The round worm infection can be controlled through adherence to the following measures:
  • Through safe pasture use such as resting pasture for more than 10 weeks will reduce the number of infective larvae on pasture or separate grazing of young and mature animals in grazing paddocks or mixed grazing of different species of animals together. The practice would assist to break the life cycle of most round worms.
  • Better grazing management should avoid overstocking of animals on a particular paddock, gazing management should also ensure that there is alternative grazing areas, should also avoid damp grazing areas and always ensure that there is better nutrition.


Treatment The following treatments and drugs are recommended for round worms.
  • Albendazole- Trade name valbazen should be administered orally at the end of cold season and beginning of dry season. The drug should be given at the rate of 10 mg/kg body weight
  • Fenbendazole-trade name panacur should be given at the end of cold and beginning dry season by mouth at the rate of 8 mg/kg body weight
  • Ivermectin- trade name ivomec can be used in different forms such as injection, or pour on. Should be given at the rate of between 0.2 - 0.5 mg/kg body weight as an intramuscular injection.
  • Levainsole and oxyclazanide mixed, trade name Nilzan, should be given by mouth at 0.25 ml/kg body weight
  • Albizidal antihemintica as a botanical treatment- the leaves of the tree are fermented and sieved and administered orally.
Flat Worm: Parasite taenia solium scolex
uterus
Also called the womb. It is a hollow muscular organ, which is part of the female reproductive system, where the fertilized egg is conceived and the fetus develops until birth.
Mucosa
The mucous membrane, or the thin layer which lines body cavities and passages.
Orally
Orally (P/O (Per Os) = by mouth)
cuticle
The outermost layer of the skin of vertebrates; epidermis
Reservoir
is a place where a great stock of anything is accumulated
oedema
Oedema is a condition of abnormally large fluid volume in the circulatory system or in tissues between the body's cells
Intramuscular
Intramuscular injection (I/M = into muscle tissue)